Pci neo vector promega

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells.

Following restriction, Name: pCI-neo, Type: plasmid, Supplier: Promega. Description: The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) 18 Feb 2016 The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus ( CMV) immediate-early enhancer/promoter region to promote pCI-neo Mammalian Expression Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or 2009 J. Biol. Chem. See all citations. Similar Products.

28.01.2021 Pci neo vector promega

When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation. E1711 Plasmid pCI-neo:alpha4-eGFP from Dr. Henry Lester's lab contains the insert alpha4-eGFP and is published in J Gen Physiol. 2011 Jan;137(1):59-79. This plasmid is available through Addgene. Name: pCI-neo: Type: plasmid: Supplier: Promega: Description: The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells.The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Plasmid pCI-neo-RL-ZEB1 from Dr. Greg Goodall's lab contains the insert 3'UTR of ZEB1 and is published in Nat Cell Biol.

Name: pCI-neo: Type: plasmid: Supplier: Promega: Description: The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting

The four major types of vectors are plasmids, viral vectors Promega pCI and pCI-neo Mammalian Expression Vectors pCI-neo Mammalian Expression; 20μg Contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in mammalian cells. The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a … This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418.

pCI-neo-ER vector was constructed by insertion of human ER cDNA derived from pIC-ER-F (ATCC, Manassas, Virginia, U.S.A.) in pCI-neo mammalian expression and neomycin resistant vector (Promega, Madison, Wisconsin, U.S.A.). pGL3-estrogen respon-sive element (ERE) luciferase reporter vector, pGL3-ERE, was constructed by insertion of four copies of

The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. pCI-neo Mammalian Expression Vector 20µg E1841 The pCI-neo Vector is supplied frozen at a concentration of 1mg/ml in TE buffer (pH 8.0). Storage Conditions:Store the pCI-neo Mammalian Expression Vector at –20°C. 3.

Name: pCI-neo: Description: Mammalian expression vector with CMV promoter, T7 and T3, chimeric intron; amp resistance (neomycin for mammalian selection); restriction enzyme cloning. The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. pCI-neo Mammalian Expression Vector 20µg E1841 The pCI-neo Vector is supplied frozen at a concentration of 1mg/ml in TE buffer (pH 8.0).

A β-globin/IgG chimeric intron located downstream of the enhancer/promoter region can further increase expression in the pCI Vector. Product Availability Update. Promega Manufacturing and Delivery Systems continue to be fully operational during the COVID-19 outbreak. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Convenient expression of cloned genes in vitro or in vivo. pCMVT n T™ Vector contains a CMV enhancer/promoter region, which enables strong constitutive expression in many cell types, while RNA phage promoters enable RNA synthesis in vitro. L5620, L5610 Cytokine Signaling Pathway Analysis Firefly Luciferase Vectors Promega: Alt Name: pCIneo, pCI neo: Analyze: Sequence: Plasmid Type: Mammalian Expression: Promotor: CMV: Cloning Method: Restriction Enzyme: Size: 5472: 5' Sequencing 1 Primer: T7: Bacterial Resistance: Ampicillin: Selectable Marker: Neomycin: Notes: This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells.

Enhancer/Promoter Regions The CMV immediate-early enhancer/promoter region present in the pCI-neo The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G418. Printed in USA. Revised 9/09.

Promotor: CMV. Cloning Method: Restriction pCI-neo. Mammalian cell expression vector with the CMV promoter and a neomycin (G418) resistance marker. To see this Sequence Author: Promega. Promega pCI and pCI-neo Mammalian Expression Vectors.

pAdVAntage™ Vector. 哺乳動物細胞に発現ベクターとともに導入することによりタンパク質発現を促進. E1711 Promega pCI and pCI-neo Mammalian Expression Vectors pCI-neo Mammalian Expression; 20μg Contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in mammalian cells. Briefly, a 895-bp fragment corresponding to nucleotides 162-1056 in the Elovl3 cDNA was cloned into a pCI-neo vector (Promega), appropriately linearized, and in vitro transcribed to obtain antisense and sense probes.

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The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression.

哺乳動物細胞に発現ベクターとともに導入することによりタンパク質発現を促進.

Mar 08, 1999

pCI-neo. Mammalian cell expression vector with the CMV promoter and a neomycin (G418) resistance marker. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer. Product Availability Update. Promega Manufacturing and Delivery Systems continue to be fully operational during the COVID-19 outbreak.

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G418. Printed in USA. Revised 9/09.